Marzieh Ebrahimi; Amin Bigham-Sadegh; Iraj Karimi; Farzaneh Hosseini
Abstract
Background: Bone grafts have been used to stimulate the fracture healing in orthopaedic surgery. Allograft and xenograft are more used because of auto graft use problems. Royal jelly is the principal food source of the queen honey bee. Royal jelly has pharmacological activities such as anti-bacterial, ...
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Background: Bone grafts have been used to stimulate the fracture healing in orthopaedic surgery. Allograft and xenograft are more used because of auto graft use problems. Royal jelly is the principal food source of the queen honey bee. Royal jelly has pharmacological activities such as anti-bacterial, antioxidant, antitumor and anti-inflammatory effects. This research was designed to evaluate the effects of royal jelly on bone healing and restoration in the rabbit model.Materials and Methods: In this study, we used 10 New Zealand white rabbits with an approximate weight of 2 kg. They were divided into two groups (N=5) midradius bone defect created and filled with royal jelly and in the second group (N=5) the defected filled with nothing. The radiological evaluation performed on days 14th, 28th and 42nd after the operation. Histopathological evaluation was done on 42 days after the operation.Results: Radiological result has not shown the significant difference in days 14th and 42nd (p>0.05) and only on day 28, there was a meaningful division (p<0.05). There was no significant difference between the two groups in histopathological evaluation.Conclusion: This study indicates using of royal jelly could accelerate bone healing process in rabbit and has the potential for use in medicine.
Iman Hafar; Amin Bigham-sadegh; Amin Nematollahi; Iraj Karimi; Saeid Lotfi
Abstract
Background: Acceleration of bone healing is one of the most challenging issues in orthopedic science. This study aimed to evaluate bone healing process with the application of fish bone powder and human lyophilized platelet (prepared with a novel protocol) in the rabbit animal model. Materials and Methods: ...
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Background: Acceleration of bone healing is one of the most challenging issues in orthopedic science. This study aimed to evaluate bone healing process with the application of fish bone powder and human lyophilized platelet (prepared with a novel protocol) in the rabbit animal model. Materials and Methods: This study was carried out on 20 male New Zealand white rabbits (12 month old), divided into four equal groups as control, fish bone powder, lyophilized platelet and a combination of fish bone powder and lyophilized platelet. After exposing the radius, a bone segment (10 mm) was cut from the bone, and the empty space was left empty in the control group but filled with the mentioned biomaterials in other groups. Radiographs of each rabbit were taken on the 14th, 28th, 42nd, and 56th post-operative days to evaluate bone formation, union and remodeling of the bone defect. All animals were euthanized on the 56th post-operative day for histopathological evaluation. Results: Radiological evaluation showed a significant difference between the lyophilized platelet group (P=0.02) and the control (P=0.007) and the fish bone powder (P=0.005) on 56th post-operative day, where the lyophilized platelet group was superior, compared to other groups. Moreover, the histopathological evaluation revealed a significant difference between the control group (P=0.01), the fish bone powder (P=0.03) and lyophilized platelet group (P=0.01), where treatment groups were superior, compared to the control group on 56th post-operative day. Nonetheless, there was no evidence of graft rejection in all groups.Conclusion: According to the results of the study, using lyophilized platelet could accelerate the bone healing process in rabbit and has the potential for use in medicine.
Amin Bigham-Sadegh; Iraj Karimi; Farzaneh Hoseini; Hamid Moradi
Abstract
Background: Nowadays, bone grafting is used in both human and veterinary orthopedics to stimulate fracture healing, accelerate joint ::::union::::, and to restore bone defects. In such procedures, orthopedic surgeons are searching to favorable substitute for autograft bone. The purpose of this study ...
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Background: Nowadays, bone grafting is used in both human and veterinary orthopedics to stimulate fracture healing, accelerate joint ::::union::::, and to restore bone defects. In such procedures, orthopedic surgeons are searching to favorable substitute for autograft bone. The purpose of this study is evaluation of theranekron and hydroxyapatite effects on bone healing in rabbit model.
Methods: First, 20 rabbits were prepared and they were divided into four groups of five. In the group of theranecron, theranecrone was injected to the bone defected site on days 3, 7, and 10 after surgery and one of the group was left empty as control group and in the third group combination of the theranekron and hydroxyapatite was filled in the defected site and in the final group hydroxyapatite was implanted alone. Radiographs from rabbit's hand after surgery were taken on days 14, 28, 42, 56 in lateral view. At the end of the study, histopathological samples were taken from injured area of radius.
Results: In radiographical and histopathological evaluations the group of theranekron-hydroxyapatite had the best performance in healing criteria and the control group was the weakest group.
Conclusion: theranekron group and theranekron –hydroxyapatite groups were better than two other groups in bone healing criteria by radiological and histopathological evaluation.
Amin Bigham-Sadegh; Siavash Sharifi; Iraj Karimi; Ahmad Oryan; Mina Maleki
Abstract
Background: Orthopedic surgeons are trying to find best substitutes for bone grafting in human and veterinary medicine. Bone autografts are still as a golden standard in comparison with other bone grafts. Diced ear cartilage from dog and rabbit have been used on bone healing previously, in the ...
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Background: Orthopedic surgeons are trying to find best substitutes for bone grafting in human and veterinary medicine. Bone autografts are still as a golden standard in comparison with other bone grafts. Diced ear cartilage from dog and rabbit have been used on bone healing previously, in the present study effects of rabbit and bovine rib cartilages will evaluated in bone healing of rabbit bone defect model.
Method: In this study, 20 adult rabbits weighing approximately 2 kg were used. The rabbits were randomly divided into 4 groups. The bone segment was removed from the mid radial bone. In the first group (N = 5) in the gap segement of bovine rib cartilage was implanted. In the second group (N = 5) in the gap, segement of rabbit rib cartilage was implanted. In the third group (empty control group), (N = 5) the defect was left without implantation. Finally, the fourth group (autograft Group), (N = 5) the defect was filled with a same harvested bone. The skin and muscles were sutured routinely. X-rays were taken on 14th , 28th , 42nd and 56th postoperative days.
Result and conclusion: After 8 weeks bone samples were taken from healed area for for histopathological evaluation. The results of our study indicate allogenic and xenogenic cartilage acted almost like autograft groups and were better than empty group.
Iraj Karimi, PhD; Amin Bigham-Sadegh, PhD; Ahmad Oryan, PhD; Zohre Rahmani, DVM
Abstract
Background: Rupture of tendons is a common injury. The outcome of surgical repair of injured tendon is often unsatisfactory. At best, the restored tendon is about half of its initial mechanical properties. In this study the effect of zenologous and autologous platelet gel were compared in rabbit ...
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Background: Rupture of tendons is a common injury. The outcome of surgical repair of injured tendon is often unsatisfactory. At best, the restored tendon is about half of its initial mechanical properties. In this study the effect of zenologous and autologous platelet gel were compared in rabbit model.
Methods: 45 rabbits in the age range of 7 months to 1 year old were divided into autologous, zenologous and control groups. Superficial digital flexor tendon was cut transversely and then sutured with Mayer stitch pattern. Then 5cc of either aotologous or zenologous platelet gel was injected to the incision area. The control group had no material injection. On 7th, 14th, and 28th post-operative days, five rabbits of each group were euthanized and tendons were harvested for histopathological and biomechanical evaluations. The results were analyzed statistically.
Results: Biomechanical factors were significantly superior in the autologous and zenologous groups than the control group. In histopathological examination the autologous groups showed a significant difference in fibroblast maturation in all the tested weeks. The collagen fiber alignment at 7th postoperative day and collagen accumulation on 7th and 28th postoperative days were superior in autologous compared with zenologous and control groups.
Conclusions: Utilization of zenologous platelet gel has a positive effect on tendon healing, but not as good as autologous platelet gel.
